Artificial RNA Interference on <i>Frankliniella occidentalis</i><italic/>

LI Heng; ZHOU Zhi-en; CHEN Yong; LU Cheng-cong; ZHANG Xiang-qin; WANG Liang; CHEN Yi-xin; TIAN Hou-jun; LIN Shuo; ZHANG Jie; YOU Yong; WEI Hui

doi:10.19303/j.issn.1008-0384.2019.10.010

Volume 34 Issue 10

Oct. 2019

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Article Navigation > Fujian Journal of Agricultural Sciences > 2019 > 34(10): 1179-1184

LI H, ZHOU Z E, CHEN Y, et al. Artificial RNA Interference on Frankliniella occidentalis [J]. Fujian Journal of Agricultural Sciences，2019，34（10）：1179−1184. doi: 10.19303/j.issn.1008-0384.2019.10.010

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LI H, ZHOU Z E, CHEN Y, et al. Artificial RNA Interference on Frankliniella occidentalis [J]. Fujian Journal of Agricultural Sciences，2019，34（10）：1179−1184. doi: 10.19303/j.issn.1008-0384.2019.10.010

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Artificial RNA Interference on Frankliniella occidentalis

doi: 10.19303/j.issn.1008-0384.2019.10.010

LI Heng^{1, 2
,},
ZHOU Zhi-en¹,
CHEN Yong²,
LU Cheng-cong²,
ZHANG Xiang-qin^{1, 2},
WANG Liang^{1, 2},
CHEN Yi-xin²,
TIAN Hou-jun²,
LIN Shuo²,
ZHANG Jie³,
YOU Yong²,
WEI Hui^{1, 2
,
,}

1.
State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, Fujian Agriculture and Forestry University, Fuzhou, Fujian　350002, China
2.
Institute of Plant Protection, Fujian Academy of Agricultural Sciences/Fujian Key Laboratory for Monitoring and Integrated Management of Crop Pests/Fuzhou Scientific Observing and Experimental Station of Crop Pests, Ministry of Agriculture and Rural Affairs, Fuzhou, Fujian　350003, China
3.
Biotechnology and Germplasm Resource Institute, Yunnan Academy of Agricultural Sciences/Yunnan Provincial Key Laboratory of Agricultural Biotechnology/Key Laboratory for Gene Resources and Germplasm Innovation of Southwest Crops, Ministry of Agriculture and Rural Affairs, Kunming, Yunnan　650223, China

Received Date: 2019-08-28
Rev Recd Date: 2019-09-29
Publish Date: 2019-10-01

Abstract

Abstract

Objective Application of dsActin to control the proliferating, multiple-hosting, insecticide-resistant invasive western flower thrips on vegetables and ornamental crops in China was investigated. Method Synthetic dsActin or dsGFP (as control) was introduced into the 2^nd-instar nymphs of Frankliniella occidentalis (Pergande) by either membrane-feeding or microinjection. The treated thrips were collected for mRNA expression detection by RT-qPCR at 24, 48 and 72 h after the treatment. Effects of 0.5 μg·μL⁻¹ dsRNA on the body length, wing or thorax malformation, and mortality of the insects were monitored in an individual culture experiment conducted in the laboratory. Result The relative expression of Actin mRNA in the thrips was similar to that of control 24, 48 and 72 h after membrane-feeding. But the expressions in the injection group decreased to 68% in 24 h, 56% in 48 h, and 53% in 72 h. Significantly higher mortality rate (44%-98% in 24-120 h), shorter body length (90% of control), and higher bodily deformation rate (41%) were observed in the dsRNA-treated thrips than control. Conclusion The Actin mRNA expression in F. occidentalis was significantly suppressed by dsActin injection at the test level to induce malformations or death in the insects. It suggested that an in vivo RNA interference method was made available for studying the gene functions and control of thrips.
- Frankliniella occidentalis,
- RNA interference,
- membrane-feeding,
- microinjection

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References(23)

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