Abstract:
: Objective Rice grain length is one of the components of rice grain type, which directly affects grain yield. It is of great significance to mine more genes related to grain type for genetic improvement of rice grain type.
Method In this study, through map-based cloning, we employed 499 recombinant inbred lines derived from the interspecific cross between Jiafuzhan (indica) and Nipponokin (japonica) to identify quantitative trait loci (QTLs) associated with grain length traits, and its application in breeding was analyzed.
Result The results showed that a major plasmid length gene GS3 was detected in the recombinant inbred line population, and the gene was located within the physical distance of about 1851 kb. By sequencing analysis, it was found that there was only 1 base difference in this gene between Jiafuzhan and Nipponbare, and the 48th base of the second exon was mutated from C to A, resulting in premature termination of protein translation. Further analysis showed that Jiazao 1 hao and Jiafuxiangzhan bred by the researchers using the grain length gene GS3 had no significant difference in grain length, grain width and length-width ratio compared with the wild type Jiafuzhan.
Conclusion This study provides excellent genetic resources and theoretical basis for the functional study of GS3 gene and the genetic improvement of high yield and high quality of rice..
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