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2009 Vol. 24, No. 3

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Purification of Anguilla anguilla serum immunoglobulin by euglobulin precipitation
WANG Xi-long, GONG Hui, YANG Jin-xian, LIN Tian-long
2009, 24(3): 197-200.
Abstract:
The purification of serum immunoglobulin from the European eel(Anguilla anguilla) was carried out by means of euglobulin as well as ammonium sulfate precipitation.Purified by euglobulin precipitation,the resultant material analyzed by SDS-PAGE showed two distinct protein bands,indicating a higher purity of the A.anguilla serum immunoglobulin than that obtained by ammonium sulfate precipitation.The ELISA analysis showed that the serum immunoglobulin purifid by euglobulin precipitation had a higher antibody activity than that purified by ammonium sulfate precipitation.Furthermore,the Western-blot antibody activity test also yielded a positive result on the serum immunoglobulin purified by euglobulin precipitation.
Development of PCR assay for detecting avian Pasteurella multocida
SHI Shao-hua, CHENG Long-fei, FU Guang-hua, CHEN Hong-mei, WAN Chun-he, CHEN Zhen, PENG Chun-xiang, HUANG Yu
2009, 24(3): 201-204.
Abstract:
To establish a specific and sensitive detection methodology for avian Pasteurella multocida infection,PCR was applied.The 460 bp of the specific fragments was amplified from avian P.multocida genome.None could be obtained from Escherichia coli,Riemerella anatipestifer,Salmonella,Staphyloccocus aureus,Avian paramyxovirus type 1,Muscovy duck retrovirus or genome of duck.The sensitivity test indicated that a minimum of 1ngμL-1 of avian P.multocida genome DNA could be detected by the newly developed PCR method.
Bioinformatic analysis of angiotensin converting enzyme in Danio rerio
LIU Shu-ji, WU Cheng-ye, SHAN Chao, WANG Ai-rong, WANG Zong-hua
2009, 24(3): 205-211.
Abstract:
Angiotensin converting enzyme(ACE) in Danio rerio was predicted and analyzed by using bioinformatic tools.The results showed that there were two ACE genes,i.e.,wu:fb81h03 and danio:ace2,encoding 1324 and 782 amino acids,respectively,in Danio rerio genome.Wu:fb81h03 is an acidic,water-soluble,unstable mitochondria trans-membrane protein containing no signal peptide.On the other hand,danio:ace2 is an acidic,water-soluble,stable and contains a signal peptide.Both of them include a conserved domain,Pfam Peptidase_M2,indicating that they belong to M2 family proteins.Through the analysis of ESTs,these 2 genes were found to express differentially in different tissues and growth stages.Homology BLAST and phylogenetic analysis indicated that D.rerio ACE was close to ACEs from lower vertebrates(e.g.,Gallus gallus and Xenopus(Silurana) tropicalis) and higher vertebrates(e.g.,human and mouse).The homological percentage was more than 60%,suggesting that the ACE was conserved through the evolution process.
A new,high quality early Indica rice,Jiazao No.1
CAI Jin-lei
2009, 24(3): 212-216.
Abstract:
Jiazao No.1 is a new early Indica rice variety with promising qualities for promotion.It was successfully bred by means of a new breeding technology.Its quality almost reached the criteria for A-level Edible Rice set forth by the Agriculture Department of China.Its average yield was 6000-6500 kghm-2.This newly bred variety has other desirable characteristics,such as,resistance to high winds and blast diseases,steady productivity and strong adaptability.It might offer a solution to the long existing problems associated with the large grain rice and early Indica rice for the rice industry.
Dynamic models simulating root length and growth characteristics of rice
LI Juan, ZHANG Ming-qing, LIN Qiong, CHEN Zi-cong, YAN Ming-juan, KONG Qing-bo
2009, 24(3): 217-221.
Abstract:
Based on the water and soil cultivation experiments on rice and the root growth model of water spinach and lettuce,a model to describe the cycle and characteristics of the root growth of rice was established.Statistical significance of the model for 7 rice varieties was obtained.For instance,the model could predict the root length growth constant,the maximum root growth rate and length,as well as the time for their appearances.The water and soil cultivation experiments showed that all varieties retained a large root system at later stage of growth;no significant difference on root growth characteristics among the late rice varieties,except Teyou 3101;the variation coefficient of the maximum root growth rate among different species was 7.6%;and the time for the maximum root growth was about two months after sowing,suggesting an advantage of topdressing at this time for fertilization efficiency.The soil cultivation experiment showed that the root growth constant could be increased 57.2% by application of a balanced fertilization of nitrogen,phosphorus and potassium,as compared with no fertilization.The maximum root growth rate and root length were increased 61.0% and 65.4%,respectively,showing the apparent effect of the timed fertilization in promoting growth and increasing length of the roots for rice.
Combining ability and inheritability of yield and quality factors of hybrid rice
LAI Yong-hong
2009, 24(3): 222-226.
Abstract:
The combining ability and hereditary capacity of various yield and grain quality factors of the F1 hybrid rice were analyzed.The hybridization was based on p×q incomplete diallele cross(NCⅡ) design of 4 high quality sterile lines of Indica-type and 5 main popularized strong restorer lines of Indica-type(4×5).The results showed that(a) the general combining ability of all selected traits,except 1000-grain weight,showed highly significant differences;(b) the specific combining ability of all selected traits,except 1000-grain weight,had significant or highly significant differences;(c) the ratio of genotypic variance to the entire genotypic variance of the general combining ability was significantly higher than that of the specific combining ability;(d) other than gel consistency,the sterile lines play a dominant role in the traits of hybrid rice.The inheritability was estimated to be more than 70% for all traits and 90% for some.Either broad or narrow sense of the inheritability was basically same.Taking everything into account,the sterile lines,Luxiang 618A and Yixiang1A,and the restorer lines,Minghui 1259,Fu 819 and R527,were considered to be of value for breeding high quality,high yield,three-line hybrid rice.
Change in endogenous hormone contents of mango during floral differentiation after heading-back
GAO Xiao-jun, WU Xing-en, WANG Shi-yu, CHEN Chun-ling, CHENG Jing-hui, DONG Guang-ping, Peng Lei
2009, 24(3): 227-230.
Abstract:
The mango buds continue floral bud differentiation,to blossom and bear fruits in the same year after flower brands have been headed back.That observation initiated this study.The contents of ABA,GA3,IAA and ZR in the headed-back mango were determined.The results of the analysis for the buds collected from the Mango Shan Zhuang,Hua Qiao Nong Chang and Yuan Jiang Nong Chang showed that(a) during flower bud differentiation,IAA remained low in all samples;(b) ABA decreased to 225.50,397.43 and 610.31 ngg-1FW,respectively,then increased to 13016.4 ngg-1FW;(c) GA3 increased to 9375.20,6022.51 and 8924.24 ngg-1FW,respectively;(d) ZR increased to 9761.68,10764.91 and 9176.39 ngg-1FW,respectively;(e) when the flower bud differentiation was basically completed,the ABA decreased to 840.49,309.19 and 309.19 ngg-1FW,respectively;(f) at the same time,GA3 decreased to 183.41,167.63 and 187.13 ngg-1FW,respectively;(g) also,IAA decreased to 540.11,573.49 and 1166.48 ngg-1FW,respectively;and(h) ZR decreased to 308.51,330.86 and 567.79 ngg-1FW,respectively.The ratios of ABA/IAA,ZR/IAA,ABA/GA3 and ZR/GA3 peaked on February 25,then,decreased significantly.This suggested that balances between different endogenous hormones also played an important role in regulating and controlling mango's flower bud differentiation.It was concluded that by increasing the contents of ABA and ZR,or decreasing the contents of GA3 and IAA,mango flower bud differentiation could be accelerated.
Breeding of a new cutting sunflower variety,Minri 1
WU Jian-she, ZHONG Huai-qin, HUANG Min-ling, LIN Bing, YE Xiu-xian, CHEN Yuan-quan
2009, 24(3): 231-236.
Abstract:
The spontaneously mutated male individuals of the pollen abortion ornamental sunflower were used to breed the cutting sunflower,"Minri 1" by hybridizing,backcrossing and inbreeding.The new variety has an apparently different flower color from their parents.In particular,its yellow petals,green discs,black anthers,yellow pollens and flamboyant and distinct flower discs give the stable ornamental characteristics.Minri 1has a growth period of 80-120 d.Its plant height is 115-170 cm with flower diameter of 11-15 cm,petal number of 35-45,and a cut flower lifespan of 8-14 d.The vigorous,adaptable,new variety has a short growth period,can be cultivated year-round and provides a cutting output of 90000-120000 pieceshm-2.
A preliminary study on pollen Irradiation for Phalaenopsis breeding
ZHANG Yong-bo, LIAO Fu-qin, ZHONG Huai-qin, HUANG Ping-ping, LIU Tian-feng, LIU Zhi-cheng
2009, 24(3): 237-240.
Abstract:
Phalaenopsis pollens treated with 60Coγ-ray radiation were used for the experimentation.Varying doses of 60Coγ-ray radiation were applied.The results showed that 60-80 Gy were optimal for the irradiation treatment.A variety of the resultant Phalaenopsis was selected for further studies.
Residue and Safe Application of Imidacloprid on Vegetable Soybean
CAI En-xing
2009, 24(3): 241-245.
Abstract:
To determine the changes in residue and environmental safety of imidacloprid application on soybeans used as a vegetable,HPLC and field experimentation were conducted.At the same time,a safe application method of the insecticide was developed.The result showed that(a) the imidacloprid degradation followed first order kinetic,(b) the initial imidacloprid residue amount varies with method of application,(c) the degradation rates were basically the same,(d) the degradation coefficient,(︱k︱),was 0.186 3±0.014 9,(e) the DT50 was 3.5-3.9 d,and(f) the T99 was 22.9-26.0 d.The soybeans were sprayed with 3.00 g of effective imidacloprid per 667 m2 near time of harvest.The insecticide residues,3 days after the application,were all less than 1.0 mgkg-1,averaging 0.299 mgkg-1.When the spray was applied twice at 7d interval,the residues were all less than 1.0 mgkg-1 averaging 0.374 mgkg-1,5d after the second spray.The residue levels on the soybeans were all below the Japanese or American MRL requirements.
Soil testing and fertilization indices for major grain and oil crops in Fujian Ⅳ.Correlation between soil test data and fertilization model
ZHANG Jian-li
2009, 24(3): 246-250.
Abstract:
Based on a large number of field fertilization experiments on rice,sweet potatoes,potatoes and peanuts,fertilization models were established.Soil tests for alkali-hydrolyzable nitrogen,Olsen-P and available K were conducted for data to perform a regression analysis against these models.The results showed a correlation in an exponential function with F values reaching significant level.Thus,the established models were considered applicable in practice.
Microbial isolation,identification and physiology of compound bacterial manure
CAO Yi, LIU Bo, ZHU Yu-jing
2009, 24(3): 251-253.
Abstract:
The microbial flora in the compound bacterial manure were isolated,identified and the amount of the bacteria determined.The physiological characteristics of the strains were studied.In the compound bacterial manure,there were potash germs,phosphate germs and nitrogen fixation bacteria(Azotobacter vinelandii) with considerable activities.The total counts in the manure reached 200-250 million per gram.The nitrogen fixation bacteria grew well,and exhibited high activity on a modified Dobereiner culture medium.The optimum growth conditions were found to be 20-32℃ and pH 7.5.
Effect of lead stress on membrane and antioxidant systems in Vetiveria zizanioides leaves
LIN Jie, KE Jin-lian, WEI Dao-jun
2009, 24(3): 254-257.
Abstract:
Effects of lead stress on the membrane and antioxidant systems in Vetiveria zizanioides leaves were studied.The results showed that the chlorophyll(Chl) content and SOD,POD and CAT activities increased,as compared to the control,when lead concentration was less than 500 mg L-1.These stress indices decreased when lead concentration was greater than 500 mg L-1.Meanwhile,the chlorophyll a/chlorophyll b ratio remained relatively constant.With increasing lead stress,the relative membrane permeability and MDA content increased considerably.A positive correlation between them was observed.The AsA content varied significantly,while GSH remained practically unchanged.And,the biomass of V.zizanioides decreased significantly at lead concentration of 1000 mgL-1 and 2000 mgL-1.
A preliminary study on formaldehyde absorption and protective enzyme activity of Sansevieria trifasciata
LIN Li-xian, XIE Zhi-nan, SU Ming-hua, LAI Rui-yun, ZHONG Zhan-hua, LI Hui-hua
2009, 24(3): 258-261.
Abstract:
Reaction of Sansevieria trifasciata plant to formaldehyde pollution in a closed container was studied.Various formaldehyde concentrations were applied and the corresponding protective enzyme activities in the S.trifasciata plant determined.The results showed that at formaldehyde concentration of 0.347 mgm-3,0.431 mgm-3,0.543 mgm-3,0.672 mgm-3 and 0.884 mgm-3,the absorption rates of formaldehyde by the plant was 14.98%,20.64%,30.76%,39.43% and 45.14%,respectively.No significant correlation between the formaldehyde concentration in the container and that in the plant were found.On the other hand,the formaldehyde content in the plant varied with treatment time.The protective enzyme(i.e.,POD,SOD and CAT) activities in the plant showed no significant difference,when the formaldehyde concentration was varied.The results suggested that the formaldehyde absorbed by S.trifasciata did not accumulate in the plant,leaving no significant residual effects on it.
Porosity of biomass chars studied by using nitrogen adsorption method
LIN Xiao-fen, ZHANG Jun, YIN Yan-shan, SHENG Chang-dong
2009, 24(3): 262-265.
Abstract:
Porosities of 4 biomass chars of rice hull,tree leaf,cornstalk and cotton stem were studied using the N2 adsorption method.The special surface area(SSA) and pore size distribution were obtained for the samples.The results showed that there were significant differences in SSA and pore size distribution among the biomass chars.The largest SSA was 242.21 m2g-1,while the smallest 0.81 m2g-1.At the pyrolysis temperature of 850℃,the pore size distributions of rice hull,tree leaf and cornstalk char peaked in the micropore and the mesopore ranges.In contrast,the cotton stalk char had only one peak in the mesopore range.The pyrolysis temperature considerably affected the porosity.Synchronous heating resulted in larger SSA and more fine pores than asynchronous condition.In this study,it was found that chars obtained at 600℃ or 850℃for rice hull and 850℃ for tree leaves had the largest SSA.Therefore,they were considered more suitable to be used as adsorbents.
Genetic diversity of 18 ryegrass varieties determined by RAPD analysis
DONG Xiao-ning, ZHANG Xiao-pei, LI Wen-yang
2009, 24(3): 266-269.
Abstract:
RAPD molecular-marker technique was used to detect the genetic diversity among 18 ryegrass germplasms.Their genomic DNA were tested with RAPD using 26 10-mer primers screened from 110 arbitrary 10-mer primers.A total of 197 RAPD bands were obtained,in which 187 were polymorphic.The average percentage of polymorphic bands was 94.9%.The variation range of genetic distances was from 0 to 0.846 7.By a UPGMA cluster analysis on the RAPD molecular-markers,these ryegrass varieties were divided into three groups:(1) Yatsyn,Tetragold,Pandora,Abundant,Angus,Italian Ryegrass,Barwoltra,Normal Annual Ryegrass,Green Spirit,Normal Diploid and Triticale;(2) Peak,Cailente,Pinnacle,Primer and Bright Star;and(3) Dong Mu 70.
Advance in research on effects of land use and management on soil carbon stock and fractions
WANG Feng, WENG Bo-qi, WANG Yi-xiang
2009, 24(3): 270-278.
Abstract:
This paper reviews the research results on the fractionation of soil organic carbon.A focus was placed on the dynamics of the decomposition,accumulation,content,storage and allocation proportion of soil organic carbon and its fractions under different land uses and management.The information should aid in formulating appropriate measures to reduce greenhouse gas emission.